fluorescence protein coding sequence  (Bio-Rad)

Journal: Scientific Reports

Article Title: The orphan solute carrier SLC10A7 is a novel negative regulator of intracellular calcium signaling

doi: 10.1038/s41598-020-64006-3

Figure Lengend Snippet: Expression pattern of SLC10A7 transcript variants v2 and v4 in different human tissues. Expression analysis was performed on a commercial major human tissue cDNA panel with primers theoretically allowing amplification of all different transcript variants (see Supplementary Table ). Primers were designed to bind at exon boundary 3/4 (forward) and at exon 12 (reverse). Amplicons were separated on a 2.5% agarose gel. Only transcript variants v2 (amplicon size: 807 bp) and v4 (amplicon size: 918 bp) were detected. Individual bands were excised from the gel and sequence verified by DNA sequencing. Images were cropped from different gels for better clarity. The full-length gels are depicted in Supplementary Fig. .

Article Snippet: Briefly, the flexible linker protein sequence GGGGSGGGGSGGGG, followed by the cDNA sequence coding for the monomeric red fluorescent protein mScarlet were added to all constructs via DNASTAR 15.0 SeqBuilder Pro and were synthesized by Biocat (Heidelberg, Germany) into the pcDNA3.1(+) expression vector (Thermo Fisher Scientific).

Techniques: Expressing, Amplification, Agarose Gel Electrophoresis, Sequencing, DNA Sequencing